Present study has been conducted to evaluate antiasthmatic activity of 3 different plants and its comparison with formulation developed from that plants. Antiasthmatic activity of all hydroalcoholic extracts were assessed in-vitro by study on albino rat mesenteric mast cell degranulation by compound 48/80 and protection by test samples and in-vivo by observing respiratory symptoms and analyzing total and differential leukocyte count in BAL fluid and in blood cell. In-vitro study represents that rat mesentery treated with compound 48/80 showed remarkable disruption of mast cells. While groups treated with Dexamethasone and hydroalcoholic extract of Nardostachys Jatamansi rhizome, Ephedra gerardiana Stem and Terminalia belerica fruit showed less disruption of mast cells and having capacity to protect mast cells degranulation. Hydroalcohic extract of churna (mixture of 3 plants) having more capacity to protect mast cells degranulation and having more numbers of intact mast cells as compared to individual plants. Horse serum sensitized rats showed induced anaphylaxis reaction and mortality. Also the numbers of total leukocyte count as well as differential leukocyte count particularly eosinophil count are significantly elevated. The treatment with test drugs showed a significant (P<0.001) decreased in anaphylaxis reaction. Also decreased the elevated levels of total and differential leukocyte numbers and protect the lung. Present study reveals that the hydroalcoholic extracts of Nardostachys Jatamansi rhizome, Ephedra gerardiana Stem and Terminalia belerica fruit could afford a significant antiasthmatic activity and hydroalcoholic extract of Churna having better antiasthmatic activity as compared to individual plants.
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